The regulation of the acid-base balance in Lash Growth cells is essential for proper cellular homeostasis.Disturbed acid-base balance directly affects cellular physiology, which often results in various pathological conditions.In every living organism, the protein family of carbonic anhydrases regulate a broad variety of homeostatic processes.Here we describe the identification, mapping and cloning of a zebrafish carbonic anhydrase 5 (ca5) mutation, collapse of fins (cof), which causes initially a collapse of the medial fins followed by necrosis and rapid degeneration of the embryo.These phenotypical characteristics can be mimicked in wild-type embryos by acetazolamide treatment, suggesting that CA5 activity in zebrafish is essential for a proper development.
In addition we show that CA5 regulates acid-base balance during embryonic development, since lowering the pH can compensate DVD Parts for the loss of CA5 activity.Identification of selective modulators of CA5 activity could have a major impact on the development of new therapeutics involved in the treatment of a variety of disorders.